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The results of analytical HPLC after reaction with different enzyme concentrations are shown as follows: (A), no enzyme; (B) 25 nM PimD; (C) 50 nM PimD; (D) 100 nM PimD. Reactions were carried out for 30 min. Chromatographic peaks corresponding to 4,5-de-epoxypimaricin and pimaricin are indicated by arrows.
