Figure 2. Cross-linking of purified VDAC.

Triton X-100- and LDAO/β-OG-purified VDACs (0.2–0.3 mg/ml) were incubated for 10 min at 30 °C in 10 mM Tricine, pH 8.3 or pH 8.6 (DMS), with the cross-linking reagent, EGS (100 μM), DSP (50 μM), DMS (4 mM), DFDNB (50 μM), Sulpho-EGS (100 μM), and subjected to SDS/PAGE and immunostaining. The VDAC-crosslinked products are indicated.