Figure 1. Specific interaction of Ypa1 or Ypa2 with different PP2A-like phosphatases.
(A) Different PP2A-like phosphatases were IVTT as [35S]methionine-labelled proteins and used in GST-Ypa1 and GST-Ypa2 pull-down assays as described in the Materials and methods section. (B) Different PP2A-like phosphatases were overexpressed in different yeast strains as HA-tagged proteins and analysed by Western blot with anti-HA tag antibodies. The top panel shows the expressing level of the HA-PP2A-like phosphatases in 5 μl of cell-free extracts. In this case, Western blot of HA-Sit4 and HA-Ppg1 needs a longer exposure time (2 min versus 15 s) to detect the expressed HA-tagged proteins. The middle and the lower panels show the GST-Ypa1 and GST-Ypa2 pull-downs respectively of these yeast extracts (exposure time 15 s).