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GST-Tap42 pull-downs of (A) IVTT [35S]methionine-labelled PP2A-like phosphatases or (B) IVTT [35S]methionine-labelled Pph22 with (+) or without (−) bacterially expressed and purified His-Ypa1 or His-Ypa2 in the presence or absence of 20 μM rapamycin (R) as described in the Materials and methods section.
