Skip to main content
. 2024 Aug 26;15(8):621. doi: 10.1038/s41419-024-06996-w

Fig. 2. BRG1 promoted the proliferative ability of B-ALL cells in vitro.

Fig. 2

A Three ALL cell lines (SUP-B, RS4:11 and Nalm-6) and three AML cell lines (MV4-11, U937 and THP-1) were analysed via western blotting. Nalm-6 and SUP-B15 cells were transfected with lentiviral vectors encoding puromycin-inducible control ShRNA (Sh-Ctrl) or BRG1 ShRNA (Sh-BRG1), whereas RS4:11 cells were transfected with control (vector) or ectopic BRG1 (LV-BRG1). Puromycin was added to induce shRNA and over-expression virus vector expression for 7 days. Of the three ShRNAs that were used to suppress SMARCA4 expression, SMARCA4#3 was selected for subsequent experiments. Nalm-6 cells (B), SUP-B15 cells (C) and RS4:11 cells (D) were verified via western blotting and qRT-PCR. E Cell growth was assessed using the CCK-8 assay at 0, 24, 48, and 72 h. Cells were cultured in 6-well plates using soft agar for 14–18 days. F The proliferative ability of BRG1-knockdown or BRG1-overexpressing B-ALL cell lines was assessed using colony-forming assays. G Sample micrographs on the left and measurement of EdU incorporation on the right (scale bar = 200 μm). All experiments were repeated three times independently. Data are expressed as the mean ± standard error of the mean (*P < 0.05; **P < 0.01; ***P < 0.001).