Skip to main content
. 2024 Aug 26;15(8):621. doi: 10.1038/s41419-024-06996-w

Fig. 3. BRG1 enhances cell proliferation and resists apoptosis in B-ALL cells.

Fig. 3

Cell cycle analyzed by PI labeling. Flow cytometry (top) was used to analyse the cell cycle of BRG1-knockdown (A–B) or BRG1-overexpressing (C) B-ALL cell lines. The bottom image shows cell cycle analysis. LV-BRG1 cells were treated with a vehicle (dimethyl sulfoxide, DMSO) or PFI (100 nM) for 24 h. D, E Apoptosis of Sh-BRG1, LV-BRG1 and LV-BRG1 with administration cells was analysed via 7-ADD/annexin V labelling. Apoptosis rate is equal to the sum of the frequencies of the two right quadrants (which correspond to early apoptosis (bottom) and late apotosis/necrosis (upper). F Western blotting was used to identify markers of proliferation and apoptosis in two BRG1-knockdown and one BRG1-overexpressing B-ALL cell lines. β-actin functioned as the control for loading. G–I Histogram demonstrating the relative grey values. All experiments were repeated three times independently. Data are expressed as the mean ± standard error of the mean (*P < 0.05; **P < 0.01; ***P < 0.001).