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Summary of protein expression and purification following expression in BL21(DE3) as His6 N-terminal fusion proteins. Lane 1, cell pellet; lane 2, crude lysate; lane 3, Invitrogen SeeBlue Plus2 pre-stained standard; lane 4, inactive recombinant cABC I; lane 5, active recombinant cABC I (altered via site-directed mutagenesis). Sizes are indicated in kDa to the right of the gel.
