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Hanes representations of recombinant cABC I on C4S (●), C6S (○) and DS (▼). Inset, a comparison of enzyme activity on 1 mg/ml C6S substrate in 50 mM Tris buffer, pH 8.0, with 50 mM sodium acetate. Expression of the original clone (▲) results in a protein with negligible activity; expression of the clone manipulated to conform to the desired sequence (△) results in robust enzyme activity.
