Figure 3. The 5′-flanking sequence from the mouse SREBP-1a promoter up to the initiator methionine residue in the mRNA (−2521 to +29 relative to the mRNA start site) was fused to the firefly luciferase-coding sequence in pGL3 and analysed for promoter activity in HEK-293T cells as described in the Materials and methods section.

Serial deletions were made and also fused to luciferase as indicated. Each construct was analysed in triplicate and the average values relative to an internal control (CMV-β-gal) were calculated and plotted as fold activation above the value obtained for the empty pGL3 vector analysed in parallel.