Figure 5. Functional analysis of binding sites for Sp1 and NF-κB in the SREBP-1a promoter.

DNA containing the wild-type or indicated mutant versions of the mouse SREBP-1a promoter were fused to luciferase and analysed for promoter activity as described in Figure 3. N denotes a mutation in the putative NF-κB site. All constructs were analysed in triplicate and average values are plotted as fold activation above the value obtained for the empty pGL3 vector.