Table 5.

Concentrations of inositol phosphates, myo-inositol, and N-acetylneuraminic acid (Neu5Ac) in the distal ileum content and feces of growing pigs and urinary excretion of myo-inositol (µmol/g DM if not otherwise stated)¹

Ca source	Ca formate		Ca carbonate
Exogenous phytase, FTU/kg of diet	0	1,500	0	1,500	1,500
Formic acid, g/kg of diet	0	0	0	0	8.0	P-value
Item	Diet 1	Diet 2	Diet 3	Diet 4	Diet 5	SEM	Diet 4 vs. 5	Ca source	Phytase	Ca source × Phytase
Ileum digesta2
InsP6	35.4	8.9	34.5	7.8	5.4	0.99	0.001	0.191	<0.001	0.372
Ins(1,2,4,5,6)P5	2.5a	0.9b	2.6a	0.7c	0.5	0.08	0.001	0.203	<0.001	0.012
Ins(1,2,3,4,5)P5	1.4a	1.5a	1.5a	1.1b	0.7	0.10	0.001	0.070	0.050	0.002
Ins(1,2,3,4,6)P5	0.7	n.d.	0.6	n.d.	n.d.	0.03	–	0.327	–	–
Ins(1,2,5,6)P4	0.4c	3.2a	0.4c	2.5b	2.2	0.15	0.230	0.094	<0.001	0.019
Ins(1,2,3,4)P4	0.2c	4.1a	0.3c	3.3b	2.8	0.20	0.088	0.300	<0.001	0.009
InsP3x3	n.d.	8.4	n.d.	7.5	8.7	0.62	0.113	0.216	–	–
Ins(1,2)P2	n.d.	4.1	n.d.	5.3	8.8	0.63	0.001	0.081	–	–
Myo-inositol	2.2	7.9	2.6	9.6	11.4	1.05	0.031	0.102	<0.001	0.306
Neu5Ac, g/kg DM	0.28	0.26	0.28	0.26	0.26	0.03	0.824	0.640	0.007	0.704
Feces2
InsP6	2.4	1.7	2.3	1.6	1.2	0.45	0.344	0.840	0.088	0.766
Ins(1,2,4,5,6)P5	0.7	0.2	0.6	0.2	n.d.	0.05	–	0.227	<0.001	0.137
Myo-inositol	0.9	<LOQ	0.7	0.4	<LOQ	0.10	–	0.328	0.004	–
Urine
Myo-inositol, µg/d4	18.9	36.2	23.1	33.0	40.3	9.57	0.184	0.453	<0.001	0.815

¹Least square means based on 8 observations per diet.

²Inositol phosphate isomers not mentioned here were not detectable (n.d.) or below the limit of quantification (< LOQ).

³A discrimination was not possible because of co-elution, at least one of the following isomers was present: Ins(1,2,6)P₃, Ins(1,4,5)P₃, Ins(2,4,5)P₃.

⁴Least square means based on 7 observations per diet, as animal no. 3 had a 4.3 higher urinary excretion of myo-inositol than the mean of all other pigs.

^a,b,cWithin a row, means of diets 1 to 4 without a common superscript significantly differ (P ≤ 0.05).