Figure 3.

Functional marker expression associated with exhaustion is higher in nonresponders in both the periphery and the BM microenvironments. (A) Staining of representative populations of interest (peripheral CD8, CD4, and CD3 T cells). (B) Frequency of PD-1⁺/CD8 T cells, CD38⁺/CD4 T cells, and PD-1⁺TIM-3⁺/CD3 T cells in the periphery at baseline in patients treated with the RP2D assessed by flow cytometry. (C) Frequency of PD-1⁺/CD8 T cells, CD38⁺/CD4 T cells, and PD-1⁺TIM-3⁺/CD3 T cells in the BM at baseline in patients treated with the RP2D assessed by flow cytometry. Groups were compared using the Wilcoxon rank-sum test.