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. 2005 Feb 8;386(Pt 1):191–200. doi: 10.1042/BJ20040775

Figure 2. Detection of MZF-1–FHL3 interaction by immunoprecipitation.

Figure 2

c-Myc-tagged MZF-1 (amino acids 1–217) and HA-tagged FHL3 were produced by the in vitro transcription/translation method. A mixture of them was subjected to immunoprecipitation with anti-c-Myc or anti-HA antibody followed by blotting with anti-c-Myc and anti-HA antibodies. c-Myc-tagged p53 and HA-tagged SV40 large T-antigen were used as controls. Lane 1, BD-c-Myc-MZF-1 (amino acids 1–217)/AD-HA-T; lane 2, BD-c-Myc-MZF-1 (amino acids 1–217)/AD-HA-FHL3; lane 3, BD-c-Myc-53/AD-HA-T.