Skip to main content
. 2005 Feb 8;386(Pt 1):191–200. doi: 10.1042/BJ20040775

Figure 3. Determination of the FHL3-interacting region on MZF-1.

Figure 3

FHL3 as a fusion to AD and various deletion mutants of MZF-1 fused to BD were co-expressed in yeast AH109 cells. The co-transformed cells were examined for their ability to grow on −His/−Ade medium. When both of the two reporter genes, HIS3 and ADE2, are expressed, the cells can grow on the medium, which represents the interaction between FHL3 and the deletion mutant of MZF-1; −, no growth was observed; +, growth was observed on −His/−Ade medium; ++, significantly more growth was observed than +.