Table 1. Examination of autonomous activation.

Full-length and deletion mutants (containing amino acids 1–72, 1–141, 1–217, 185–285 or 249–485) of human MZF-1 as a fusion to GAL4 BD were expressed in yeast AH109. The activity of α-galactosidase, a secreted enzyme encoded by the MEL1 gene under the control of a GAL4-responsive promoter, was detected on SD−Trp/+X-α-Gal medium. pCL-1 (Clontech) encoding full-length GAL4 and an empty vector pGBK were used as positive and negative controls respectively.

	α-Galactosidase activity
Full-length MZF-1	−
MZF-1-(1–72)	−
MZF-1-(1–141)	−
MZF-1-(1–217)	−
MZF-1-(185–285)	+
MZF-1-(249–485)	−
pCL-1	++
pGBK	−