Figure 1.

Spleen dissociation and spleen plasma clearance

(A) Examples of two leukemic spleens (#009 and #029) outside and inside gentleMACS C tubes with different level of PBS based on spleen size.

(B) Spleen disruption program running at the gentleMACS machine.

(C) GentleMACS C tubes with leukemic spleens after program M_spleen_02_01 (left photo) and subsequent program M_spleen_03_02 (right photo).

(D) Filtering of the dissociated spleen in a 100 μm cell strainer (left photo) and pellet plus supernatant, marked with an arrow, after the first centrifugation at 400 g (right photo).

(E) Example of the 15 mL conical tubes after each of the serial centrifugations, and setting of the filtering of the cleared supernatant.