Figure 2.

Small EV isolation from the cleared spleen plasma

(A) Representative pellet of sEV after the first ultracentrifugation at 110,000 g for 70 min.

(B) Building of the 1 mL syringe in combination with a p200 tip and a p1000 tip to resuspend the sEV pellet.

(C) Laying process on the 17% OptiPrep (cushion). Arrows indicate the interphase formed.

(D) Pale ring, showing the interphase after the cushion ultracentrifugation, containing the sEV (indicated with an arrow). Different intensities of the pale ring may be observed in different preparations. In the last picture, sEV were pre-stained with a membrane dye.