Figure 3.

Small EV characterization

(A) Western blot (WB) depicting the enrichment of EV-specific biomarkers such as Alix, TSG-101, CD63 and CD81, from 4 different leukemic sEV preparations and whole cells.

(B) Representative electron microscopy images of purified leukemic sEV.

(C) HSNE clustering analysis of MB488+ LME-sEVs based on positivity for CD63 and CD81, measured by bead-free Flow Cytometry.

(D) Tunable resisting pulse sensing (TRPS) results showing the particle-size distribution profiling of purified leukemic sEV, obtained from the Exoid machine (Izon Science).