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. 2024 Aug 5;5(3):103244. doi: 10.1016/j.xpro.2024.103244
REAGENT or RESOURCE SOURCE IDENTIFIER
Biological samples

Eμ-TCL1 mouse (above 50 weeks of age) spleen

Chemicals, peptides, and recombinant proteins

OptiPrep STEMCELL Technologies 07820
BioTracker MemBright 488 live cell dye Merck SCT083
Murine anti-CD63 PE antibody, clone REA563 Miltenyi Biotec 130-108-893
Murine anti-CD81 APC antibody, clone EAT2 Miltenyi Biotec 130-102-630
Dulbecco’s phosphate-buffered saline [-] CaCl2 [-] MgCl2 Thermo Fisher Scientific 14190-094

Experimental models: Organisms/strains

Mouse: Eμ-TCL1 (on C57BL/6 background) Pr. Carlo Croce (OSU, OH, USA) MGI:3527221

Other

gentleMACS dissociator Miltenyi Biotec 130-093-235
Ultracentrifuge Optima MAX-XP Beckman Coulter 393315
MLA-55 fixed-angle rotor Beckman Coulter 393203
MLS-50 swinging-bucket rotor Beckman Coulter 367280
0.22 μL syringe filter Starlab E4780-1223
0.45 μL filter (4 mm) polyvinylidene difluoride (PVDF) EMD Millipore SLHVR04NL
Cell strainer 40 μm nylon Corning 352340
gentleMACS C tubes Miltenyi Biotec 130-093-237