Biological samples |
|
Eμ-TCL1 mouse (above 50 weeks of age) spleen |
|
|
|
Chemicals, peptides, and recombinant proteins |
|
OptiPrep |
STEMCELL Technologies |
07820 |
BioTracker MemBright 488 live cell dye |
Merck |
SCT083 |
Murine anti-CD63 PE antibody, clone REA563 |
Miltenyi Biotec |
130-108-893 |
Murine anti-CD81 APC antibody, clone EAT2 |
Miltenyi Biotec |
130-102-630 |
Dulbecco’s phosphate-buffered saline [-] CaCl2 [-] MgCl2
|
Thermo Fisher Scientific |
14190-094 |
|
Experimental models: Organisms/strains |
|
Mouse: Eμ-TCL1 (on C57BL/6 background) |
Pr. Carlo Croce (OSU, OH, USA) |
MGI:3527221 |
|
Other |
|
gentleMACS dissociator |
Miltenyi Biotec |
130-093-235 |
Ultracentrifuge Optima MAX-XP |
Beckman Coulter |
393315 |
MLA-55 fixed-angle rotor |
Beckman Coulter |
393203 |
MLS-50 swinging-bucket rotor |
Beckman Coulter |
367280 |
0.22 μL syringe filter |
Starlab |
E4780-1223 |
0.45 μL filter (4 mm) polyvinylidene difluoride (PVDF) |
EMD Millipore |
SLHVR04NL |
Cell strainer 40 μm nylon |
Corning |
352340 |
gentleMACS C tubes |
Miltenyi Biotec |
130-093-237 |