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17% OptiPrep solution
| Solution A (pH 7.4) | ||
| Reagent | Final concentration | |
| Sucrose | 0.25 M | |
| Ethylenediaminetetraacetic acid (EDTA) | 6 mM | |
| Tris hydrochloride (Tris HCL) | 60 mM | |
| Working Solution (WS1) | ||
| Reagent | Amount | |
| 60% OptiPrep | 50% | 5 volumes |
| Solution A (pH 7.4) | 0.167 X | 1 volume |
| Homogenization medium (HM1; pH 7.4) | ||
| Reagent | Final concentration | Amount |
| Sucrose | 0.25 M | |
| Ethylenediaminetetraacetic acid (EDTA) | 1 mM | |
| Tris hydrochloride (Tris HCL) | 10 mM | |
Store at 4°C for up to 3 months.
