Reagents | Final concentration | Amount |
---|---|---|
Tris HCl 1 M (pH-6.8) | 0.1 M | 5 mL |
Sodium Dodecyl Sulfate (SDS) 10% | 4% | 20 mL |
Glycerol 87% | 35% | 20 mL |
Bromophenol Blue 1% | 0.01% | 500 uL |
B-mercaptoethanol | 10% | 5 mL |
Store at −20°C for up to 1 year.
Reagents | Final concentration | Amount |
---|---|---|
Tris HCl 1 M (pH-6.8) | 0.1 M | 5 mL |
Sodium Dodecyl Sulfate (SDS) 10% | 4% | 20 mL |
Glycerol 87% | 35% | 20 mL |
Bromophenol Blue 1% | 0.01% | 500 uL |
B-mercaptoethanol | 10% | 5 mL |
Store at −20°C for up to 1 year.