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. 2024 Aug 5;5(3):103244. doi: 10.1016/j.xpro.2024.103244

Sample buffer 4X

Reagents Final concentration Amount
Tris HCl 1 M (pH-6.8) 0.1 M 5 mL
Sodium Dodecyl Sulfate (SDS) 10% 4% 20 mL
Glycerol 87% 35% 20 mL
Bromophenol Blue 1% 0.01% 500 uL
B-mercaptoethanol 10% 5 mL

Store at −20°C for up to 1 year.