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. 2024 Jul 18;27(8):110537. doi: 10.1016/j.isci.2024.110537

Figure 1.

Figure 1

HyA-FMBs promote early expression of non-collagenous proteins

(A) hBMSCs/SSCs were attached to HyA-FMBs and differentiated in chondrogenic medium, forming HyA-FMB organoids, which were digested at days 1, 3, 5, and 10 for scRNA-seq analyses in (C)–(H).

(B) Toluidine blue staining of control and HyA-FMB organoids at days 1, 3, 5, and 10 of chondrogenic differentiation. Scale bars, 300 μm. Asterisks (∗) indicate HyA-FMBs.

(C–F) Gene ontology analysis showing enriched pathways in HyA-FMB organoids compared with controls at days 1 (C), 3 (D), 5 (E), and 10 (F) of chondrogenic differentiation. Analyses performed using gProfiler’s driver GO pathway analysis from global differential gene expression (logfc>0.25, p < 0.05) between control and HyA-FMB organoids. Arrows indicate pathways of interest.

(G and H) Gene expression from days 0, 1, 3, 5, and 10 of chondrogenic differentiation. These data represent integrated data within control (G) and HyA-FMB (H) datasets for an overview of gene expression over time in each condition. Boxes indicate key trends in genes of interest (see also Figure S1).