Figure 6.
Chondrospheroid transcriptomes reveal a fetal-like chondrogenic identity
(A) PCA of hiPSC, sclerotome, and chondrospheroid datasets. Each dot represents a technical replicate.
(B–E) Gene ontology analysis representing top five pathways from sclerotome (B), day 14 (C), day 28 (D), and day 42 (E) chondrospheroids.
(F) Heatmap depicting differential gene expression: top 100 differentially expressed genes from each dataset were assessed against GO pathways listed in (B)–(E), reducing the list to 103 relevant genes.
(G) PCA of hiPSC, sclerotome, and chondrospheroid datasets, which were batch-corrected and normalized using ComBat-seq to previously published datasets,42 which included 5- to 6-week-old embryonic, 17-week-old fetal, adolescent, and adult primary human chondrocytes.
(H) Hierarchical clustering analysis of sclerotome, chondrospheroid, and primary human chondrocyte42 populations (see also Figure S6; Table S2. Excel file containing additional data too large to fit in a PDF, related to Figure 6G, H, Table S3. Excel file containing additional data too large to fit in a PDF, related to Figure 6G, H, Table S4. Excel file containing additional data too large to fit in a PDF, related to Figure 6G, H).