Figure 2. Scavengers of O₂⁻ had no effect on induction of NOX1 mRNA by PGF_2α.

A7r5 cells, maintained in DMEM with 0.5% FBS for 48 h, were incubated with 100 μM MnTBAP, 10 mM tiron or 50 μM EUK-8 for 24 h in the presence of 100 nM PGF_2α. A representative autoradiograph of three experiments is demonstrated. Relative expression levels of NOX1 normalized to 28 S RNA are shown below the blot.