Figure 5. Inhibitors of the mitochondrial respiratory chain suppressed phosphorylation of ATF-1 by PGF_2α.

(A) Time course of ATF-1 phosphorylation by PGF_2α. Serum-starved A7r5 cells were incubated with 100 nM PGF_2α for the indicated times. (B) Effects of various inhibitors of the mitochondrial respiratory chain. Serum-starved A7r5 cells were pre-incubated with 500 nM rotenone, 100 ng/ml antimycin A, 2 μg/ml oligomycin, 10 μM CCCP or the indicated concentrations of DPI for 30 min, and stimulated with 100 nM PGF_2α for 5 min. Western blot analysis was performed as described in the Experimental section. Representative results of three experiments are shown. Averaged relative intensities of the bands for phosphorylated ATF-1 are shown below the blots.