Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Step | Total protein (mg) | Total activity (units)* | Specific activity (units/mg) | Yield (%) | Purification (-fold) |
|---|---|---|---|---|---|
| Seminal fluid | 5458 | 54 | 0.0096 | 100 | 1 |
| Con A–Sepharose | 135 | 58 | 0.43 | 107 | 45 |
| Zinc-chelating Sepharose | 33 | 51 | 1.6 | 94 | 163 |
| Lys-Pip | 1.1 | 27 | 24 | 50 | 2536 |
| HiTrap Q | 0.3 | 24 | 70 | 44 | 7309 |
* One unit is defined as the amount of enzyme that cleaves 1 μmol of Lys-Ala-pNA per min under the assay conditions as described in the Material and methods section.
