Figure 3. Binding of mammalian RINGO proteins to different CDKs and p27^Kip1.

Xenopus oocytes were injected with mRNAs encoding HA-tagged CDK1 (A), CDK2 (B), CDK5 (C) or p27^Kip1 (D) and incubated overnight. The oocytes were then re-injected with mRNAs encoding different Myc-tagged RINGO family members. After 10 h, lysates from 35 oocytes were immunoprecipitated (IP) with Myc antibody and then immunoblotted, together with an aliquot of the total lysates, with Myc (RINGO) and HA (CDK or p27^Kip1) antibodies.