Table 1. Targeting of reporter constructs to mitochondria.

Enzyme	Residues in fusion construct*	Targeted	Not targeted
ALAS	1–203: destabilized GFP		×
CPO	1–132: destabilized GFP	×
	68–132: destabilized GFP		×
	90–132: destabilized GFP		×
PPO	1–178: GFP	×
	1–59: GFP	×
	1–34: GFP	×
	1–17: GFP	×
	GFP: 420–477		×
FC	7–68: GFP	×
	GFP: 390–423		×

* Residues are numbered according to the Swiss-Prot entry corresponding to each enzyme (see text for details). The GFP reporter placement is shown for each construct by its position in column 2 (e.g. all of the CPO constructs are N-terminal fusions to destabilized GFP).