Figure 1. Epitope reactivity of IDS polyclonal antibody.

(A) Epitope reactivity of a sheep polyclonal antibody raised against IDS, expressed as individual peptide sequences across the IDS protein. The level of ELISA reactivity to each peptide sequence was expressed in terms of A₄₁₀ (‘optical density’). Black bars denote high-affinity epitope reactivity. (B) Epitopes detected by the sheep anti-IDS polyclonal antibody (black) mapped on to a structural representation of IDS. (C) The N-linked carbohydrate structures (grey spheres) depicted on the same structural representation of IDS (black spheres show the active site). N-linked glycosylation sites were located on the peptides of pins 4–5, 16–17, 20–21, 35–36, 40, 46–47, 73–74 and 77.