Figure 3. Thermal denaturation of IDS.

(A) Thermal denaturation profiles for IDS detected by conformation changes in the protein at different temperatures, using the exposure of the specific monoclonal antibody reactive epitopes, 2G3.2B9 (●), 7B9.1B10 (◆), 1F7.2D11 (▲) and 2D3.1F9 (■), as a detection system. Results represent the level of ELISA reactivity at each temperature and were expressed in terms of A₄₁₀. All samples were assayed in triplicate and the result presented as the mean (S.D. for all sample points was less than 0.2). (B) Percentage loss of IDS activity in response to heat denaturation.