Figure 6.

Therapeutic effects of DNDA on inhibiting tumor growth combined with PDL1 blockade in B16 tumor‐bearing C57BL/6 mice. A) Treatment schedule of DNDA and anti‐PDL1 combination therapy. B) Tumor growth curve and C) individual tumor growth curve with indicated treatment. Data are shown as Mean ± SD (n = 5), and statistical significance was calculated via two‐way ANOVA with Tukey's post hoc test, ^* p < 0. 05, ^*** p < 0.001. D) Quantitative analysis M1 macrophages (gated on L/D⁻ CD45⁺ F4/80⁺ CD11b⁺ CD206⁻ CD86⁺), the ratio of M1 to M2 macrophages (gated on L/D⁻ CD45⁺ F4/80⁺ CD11b⁺ CD206⁺) in tumor, T_CM (gated on L/D⁻ CD45⁺ CD3⁺ CD8⁺ CD62L⁺ CD44⁺) and T_EM (gated on L/D⁻ CD45⁺ CD3⁺ CD8⁺ CD62L⁻ CD44⁺) cells in tumors. Data are shown as Mean ± SD (n = 5), statistical significance was calculated via one‐way ANOVA with Tukey's post hoc test, ^* p < 0. 05, ^** p < 0.01, ^*** p < 0.001, ns means no significance.