Skip to main content
. 2024 Jun 20;11(32):2404355. doi: 10.1002/advs.202404355

Figure 2.

Figure 2

iPSC attachment to the TIPS microcarriers leads to cell proliferation and protection against anoikis. A) iPSC proliferate on the surface of TIPS microcarriers: fluorescence microscopy images of iPSC attached to TIPS microcarriers stained for nuclei (DAPI blue) and cytoskeleton (phalloidin red), after (i) 2 hours, and (ii) 24 hours of seeding. Scale bars represent 100 µm. (iii) Proliferation of iPSC seeded on TIPS microcarriers and 2D tissue culture plastic surfaces, over 7 days in culture. Attachment of cells to the TIPS microcarriers for 24 hours appears to reduce anoikis, as indicated by the expression downstream markers of apoptosis, inactive caspases 3 and 9, and active cleaved caspase 3. Western blot image of B) iPSC and C) iPSC‐CM in suspension up to 24 hours or attached to TIPS microcarriers for 24 hours. (ii) Protein expression was quantified relative to GAPDH. Hours (h), cleaved caspase 3 (cleaved C3), 24 hour attachment to the TIPS microcarriers (TIPS). Positive controls for the onset of cell death were treated with 100 µM etoposide for 4 hours. Data are presented as mean ±SD. The significance of the data was calculated two‐way ANOVA with Dunnett's post‐hoc correction. (n = 4, *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001, statistics to matched negative control).