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. 2024 Jun 25;11(32):2309307. doi: 10.1002/advs.202309307

Figure 5.

Figure 5

miR‐29b‐3p regulates the Pten‐dependent AKT/mTOR pathway. A) Western blots showing high levels of phosphorylated AKT, mTOR and S6 in retinas overexpressing miR‐29b‐3p. B) Quantification of the data in A, n = 3. C) Alignment between the binding sites of miR‐29b‐3p and Pten. D) qPCR showed a significant decrease in the level of the Pten mRNA in miR‐29b‐3p‐overexpressing cells. E) qPCR showing efficient knockdown of Pten mRNA in retinas after Pten‐specific siRNA transfection. F) Western blots showing decreased Pten protein levels in Pten‐knockdown cells, which resulted in increased levels of phosphorylated AKT, mTOR, and S6. G) Quantification of the data in F, n = 3. (*p < 0.05, **p < 0.01, ***p < 0.001).