Figure 1.

Approaches for the analysis and interpretation of the MOA of ENMs starting from toxicogenomics data. In step 0, toxicogenomics data are generated and preprocessed. In step 1, traditional methods represent molecular alterations in terms of lists of differentially expressed genes (DEGs), while other studies (including this study) model them as co‐expression networks. In step 2, previous approaches reconstruct the mechanism of action (MOA) via functional annotation or enrichment of adverse outcome pathway (AOP) associated genes, leading to an arbitrary interpretation of the retrieved functions (step 3). The proposed study allows a reconstruction of an AOP based network of alterations, requiring minimal interpretation with respect to previous approaches.