Figure 2.

Workflow converting molecular profiles in networks based on the AOP framework. Network models of the exposure (co‐expression networks or preselected edgelists) are used as input of the framework. In step 1, a topological enrichment of the adverse outcome pathway (AOP) network is performed based on the input data. In step 2, possible molecular initiating events (MIEs, blue squares) and adverse outcomes (AOs, red squares) connecting to the enriched events (KEs, green squares) are prioritized based on topological properties of the AOP network and molecular alterations. In step 3, the list of MIEs, KEs and AOs is mapped on the AOP network to form an AOP‐based interpretation of the mechanism of action (MOA). The results can be visualized as a network or in tabular form, and interpreted independently or compared with other exposure mechanisms.