Fig. 1.

Deletion of residues 2 to 7 of αSyn slows amyloid formation in vitro. (A) Amino acid sequence of αSynWT. Blue = N-terminal region; pink = NAC; and red = C-terminal region. (B) Zyggregator (24) and CamSol (25) profiles for αSyn. Red bars indicate predicted aggregation-prone and low-solubility regions, respectively. For A and B, the N7 (²DVFMKG⁷), P1 (³⁶GVLYVGS⁴²), and P2 (⁴⁵KEGVVHGVATVAE⁵⁷) regions are in gray. (C) Fibril formation kinetics of αSynWT (black) and αSynΔN7 (red). Data are normalized to maximum signal of each curve. (D) T₅₀ and (E) T_lag values for nine replicates. (F) Quantification of the insoluble fraction at the end point of ThT assays. (G) Negative-stain electron micrographs of the ThT end points for αSynWT (black) and αSynΔN7 (red). (Scale bar, 250 nm.)