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Residues 2 to 7 of αSyn are critical for lipid-mediated fibrillation. (A) Fibril formation kinetics for αSynWT in the presence of DMPS liposomes. Key indicates [DMPS]:[αSyn] ratio. Data are normalized to the maximum fluorescence intensity of the dataset. (B and C) Negative-stain electron micrograph of the ThT assay end point for [DMPS]:[αSynWT] of 8:1 and 60:1, respectively. (Scale bar, 250 nm.) (D–F), as (A–C), but for αSynΔN7.
