Table 2.
Primers used for real-time PCR analysis.
| Genea | GenBank no. | Primer sequence (5′ to 3′)b | AT, °Cc | Product size, bp |
|---|---|---|---|---|
| β-actin | XM_003124280.5 | F: TGGAACGGTGAAGGTGACAGC | 62 | 177 |
| R: GCTTTTGGGAAGGCAGGGACT | ||||
| GLUT-2 | NM_001097417.1 | F: TGGAATCAGCCAACCTGTTT | 58 | 165 |
| R: ACAAGTCCCACCGACATGA | ||||
| CAT-1 | XM_021065165.1 | F: TGCCCATACTTCCCGTCC | 60 | 192 |
| R: GGTCCAGGTTACCGTCAG | ||||
| FATP-1 | XM_021076151.1 | F: GGAGTAGAGGGCAAAGCAGG | 61 | 208 |
| R: AGGTCTGGCGTGGGTCAAAG | ||||
| ZO-1 | XM_021098896.1 | F: CAGCCCCCGTACATGGAGA | 61 | 114 |
| R: GCGCAGACGGTGTTCATAGTT | ||||
| Occludin-1 | XM_005672525.3 | F: CTACTCGTCCAACGGGAAAG | 59 | 158 |
| R: ACGCCTCCAAGTTACCACTG | ||||
| Claudin-1 | NM_001244539.1 | F: TCTTAGTTGCCACAGCATGG | 59 | 106 |
| R: CCAGTGAAGAGAGCCTGACC |
a
GLUT-2, glucose transporter 2; CAT-1, cationic amino acid transporter 1; FATP-1, fatty acid transport protein 1; ZO-1, zonula occludens-1.
b
F, forward primer; R, reverse primer.
c
AT, annealing temperature.