Table 3.

Sequence of primers and probes for selected bacteria.

Gene	Primer sequence (5′ to 3′)a	AT, °Cb	Product size, bp
Total bacteria	F: ACTCCTACGGGAGGCAGCAG	60	200
	R: ATTACCGCGGCTGCTGG
Lactobacillus	F: GAGGCAGCAGTAGGGAATCTTC	60	126
	R: CAACAGTTACTCTGACACCCGTTCTTC
	P: AAGAAGGGTTTCGGCTCGTAAAACTCTGTT
Escherichia coli	F: CATGCCGCGTGTATGAAGAA	60	96
	R: CGGGTAACGTCAATGAGCAAA
	P: AGGTATTAACTTTACTCCCTTCCTC
Bifidobacterium	F: CGCGTCCGGTGTGAAAG	60	121
	R: CTTCCCGATATCTACACATTCCA
	P: ATTCCACCGTTACACCGGGAA
Bacillus	F: GCAACGAGCGCAACCCTTGA	60	92
	R: TCATCCCCACCTTCCTCCGGT
	P: CGGTTTGTCACCGGCAGTCACCT

^aF, forward primer; R, reverse primer.

^bAT, annealing temperature.