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Schematics of the 3D protocol with glass microbeads. (a) The microbeads and cells in the Transwell®, where they self-assemble in a monolayer with a precise structure (zoom), which allows estimating the plating area. (b) After 6 h, cells and microbeads were collected and stacked on the monolayer to progressively form layers, and a drop of culture medium was added to ensure survival overnight. (c) The day after, the scaffold was stable enough to add the remaining culture medium.
