Table 3.

Pathways overrepresented in CMECs treated with scHSP

Reactome pathways	Homo sapiens (n)	scHSP (n)	scHSP (expected)	Fold enrichment	p-value
Interferon alpha/beta signaling (R-HSA-909733)	67	9	0.07	> 100	1.25E−13
Interferon signaling (R-HSA-913531)‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬	196	9	0.22	41.12	1.19E−09
Cytokine signaling in immune system (R-HSA-1280215)	823	12	0.92	13.06	3.34E−08
Interleukin-10 signaling (R-HSA-6783783)	45	3	0.05	59.70	4.20E−02
Antiviral mechanism by IFN-stimulated genes (R-HSA-1169410)	79	4	0.09	45.34	4.22E−03

Up-regulated genes (23 genes) in scHSP-treated CMECs were analysed by the Panther software based on the human database. Pathways (Reactome database) which were overrepresented (fold enrichment) were mainly related to IFN and inflammatory pathways. N: number of genes found in the human genome (Homo sapiens) or scHSP; expected: number of genes expected for a certain pathway based on the Reactome database; fold enrichment: scHSP (n)/scHSP(expected)