Fig. 3.

c-Myc and BCL2A1 synergize to prevent cell death. a) Protein expression of c-MYC and BCL2A1 in SUDHL2 and TMD8 cells upon treatment with JQ1 [1µM], ABBV-075 [SUDHL2 100nM/TMD8 300nM], ARV-825 [SUDHL2 100nM/TMD8 30nM] or dBET6 [SUDHL2 100nM/TMD8 30nM] for up to 24 h. GAPDH serves as loading control and one representative blot out of three independent experiments is shown. b) mRNA expression of c-Myc and Bcl2a1 was assessed by qRT-PCR in the same experiments. Data are shown as mean + standard deviation (SD) with n = 3. c) Silencing of c-MYC was performed using two individual siRNAs (#1 and #2) followed by analysis of c-Myc and Bcl2a1 mRNA expression at 6 and 24 h after 2nd electroporation. d-e) siRNA-mediated knockdown of Bcl2a1 and c-Myc alone and combined in SUDHL2 and TMD8 cells was performed with two pooled siRNA sequences for each target. d) Knockdown efficiency was confirmed by Western Blot 24 h after the 2nd electroporation. e) Viability of electroporated cells 6 and 24 h after the 2nd electroporation measured by AnnexinV-FITC/PI and flow cytometry [data are shown as mean + standard deviation (SD) with n = 3]. For individual and combined knockdown, the concentration of non-targeting siCtrl was adapted to match the concentration of targeting siRNA