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. 2005 Mar 8;386(Pt 3):433–443. doi: 10.1042/BJ20041071

Figure 4. The C-terminal of hARD1 is not involved in NATH–hARD1 interaction.

Figure 4

(A) Some hARD1–V5 deletion proteins are outlined. The putative acetyltransferase motif (amino acids 45–130) is dotted, whereas the putative NLS (amino acids 78–83) is grey. (B) HEK-293 cells were transiently transfected with the indicated hARD1–V5 deletion plasmids. Cells were then harvested and the lysates were immunoprecipitated (IP) with anti-V5. The immunoprecipitates were analysed by SDS/PAGE and Western blotting. The membrane was incubated with anti-NATH and anti-V5. The experiment was repeated three times.