Table 1. The binding affinities between the wild-type Ydj1 and mutants and the Ydj1 peptide substrate GWLYEIS measured by ITC studies.
The ITC studies also generated the molar binding ratios between the peptides and the Ydj1 monomer by fitting the experimental results to the standard curve. ND, not detectable by ITC.
| Ydj1 and mutants | Dissociation constant (μM) | Binding molar ratio |
|---|---|---|
| Wild-type | 12.5 | 1.06 |
| I116A | ND | ND |
| L135A | ND | ND |
| L137A | ND | ND |
| L216A | ND | ND |
| F249A | ND | ND |
| Ydj1Δ(159–188) | 24.0 | 0.91 |
| Ydj1Δ(143–206) | 58.4 | 1.26 |