Table 1. The binding affinities between the wild-type Ydj1 and mutants and the Ydj1 peptide substrate GWLYEIS measured by ITC studies.
Ydj1 and mutants | Dissociation constant (μM) | Binding molar ratio |
---|---|---|
Wild-type | 12.5 | 1.06 |
I116A | ND | ND |
L135A | ND | ND |
L137A | ND | ND |
L216A | ND | ND |
F249A | ND | ND |
Ydj1Δ(159–188) | 24.0 | 0.91 |
Ydj1Δ(143–206) | 58.4 | 1.26 |