The indicated strains were tested for growth by applying 105, 104 and 103 cells on (A) SD plates supplemented where indicated with 40 mM MnCl2 (+Mn) or (B) YPD plates supplemented where indicated with the designated concentrations of MnCl2, CoSO4, ZnCl2, or CdCl2. Cells were grown aerobically at 30 °C for 3 days. Strains used: wild-type BY4741 (WT), MY001 (mam3Δ) and BY4741 expressing MAM3 from plasmid pMY003 (WT+2μ MAM3).