Table 2. Properties of mutants of the HlyB NBD.
ND, not determined.
| Codon change | Amino acid change | Solubility | ATPase activity |
|---|---|---|---|
| AAA→ATG | Lys508→Met WalkerA | Soluble | Inactive, binds ATP |
| GAT→ATG | Asp630→Met WalkerB | Soluble | Inactive, binds ATP |
| TGT→GCT | Cys652→Ala | Soluble | Active |
| CAT→GCG | His662→Ala | Soluble | Inactive, binds ATP |
| GTG→GCG | Val548→Ala | Insoluble | ND |
| CCT→CTT | Pro624→Leu | Insoluble | ND |
| CCT→GCG | Pro624→Ser | Insoluble | ND |
| CCT→GCG | Pro624→Cys | Insoluble | ND |
| CCT→GCG | Pro624→Arg | Insoluble | ND |