Figure 1. Effect of calcium on the spectral properties of pfLamA in 7.9 M GdmCl.

(A) Near-UV CD spectra were recorded in a 1 cm quartz cuvette at 0.6 mg/ml protein concentration and (B) fluorescence spectra were recorded at 40 μg/ml protein concentration (290 nm excitation wavelength). All the spectra were recorded at 20 °C after 24 h incubation of the protein in 20 mM Tris/HCl at pH 7.4 (-----) and in 7.9 M GdmCl at pH 7.4 in the absence (—–) and presence of 40 mM CaCl₂ (……).