Table 2. Quantification of GA mRNA isoforms using a competitive RT-PCR method.

Each experiment was carried out at least in triplicate.

		GA mRNA (fg/μg of total RNA)
Isoenzyme	Cell type…	MCF7	ZR-75-1	HepG-2	Leukaemia MBMNCs	Medullar aplasia
KGA		∼50†	∼50†	∼50*†	10*	0
LGA		100	100	1000	100	1500

* P<0.01 as tested with the Mann–Whitney U test.

† The relative amount of KGA mRNA was between 10 and 100 fg/μg of RNA in MCF7, ZR-75-1 and HepG-2 cells.