Table 2. TPCK effect on the PMN respiratory burst.
Control and TPCK-treated cells were activated with PMA in the assay cuvette as described in the Experimental section. The cells that had been incubated with PMA, with or without additional TPCK treatment (last 3 rows), were assayed in the absence of PMA in the spectrophotometer cuvette. Control cells did not undergo any treatment. PMA-only and TPCK-only treated cells were tested for superoxide production immediately after the washing procedure, and were left standing at room temperature with gentle occasional shaking until the end of the second incubation for the other samples. They were all sonicated together. Other details for the various incubations are described in the Experimental section. The numbers in parentheses are the number of independent cell preparations tested.
| Activity (%) | ||||
|---|---|---|---|---|
| Cell treatment | PMA | Superoxide production (nmol/min per 106 cells) | Relative to control | Relative to PMA |
| Control | + | 9.9±2.0 (10) | 100 | – |
| TPCK | + | 1.3±0.7 (10) | 13±7 | – |
| PMA | – | 4.7±1.4 (10) | 47±14 | 100 |
| TPCK then PMA | – | 0.5±0.6 (5) | 5±6 | 11±13 |
| PMA then TPCK | – | 0.3±01 (5) | 3±1 | 6±2 |